Hach-Lange LZV937 - DR 6000 additional software enzymology tests - Handbuch (Seite 39)

English 39
D-glucose/D-fructose and D-glucose/D-fructose (S)
Procedure for D-glucose/D-fructose
1. Prepare the test according to the process specification.
2. Select STORED PROGRAMS > SELECT BY NUMBER.
3. Enter the number 2210 for the program "D-glucose". Confirm with
OK.
Enter the number 2211 for the program "D-glucose (S)" with creep
monitoring. Confirm with OK.
4. Press START to start the program.
5. Set the blank value cuvette to carousel position 1.
6. Set the sample cuvettes to carousel positions 2-7.
7. Press READ CAROUSEL.
The 3:00 timer starts. When the time on the timer elapses, two short
acoustic signals are sounded.
Extinction 1 is measured in all cuvettes. R1 is shown on the display.
8. Pipette the reagent into the blank value and sample cuvettes
according to the process specification.
9. Press READ CAROUSEL.
The 15:00 timer starts. When the time elapses, two short acoustic
signals are sounded.
Extinction 2 is measured in all cuvettes.
R2 is shown on the display.
Note: For program 2211 D-glucose/D-fructose (S) with creep
monitoring, extinction 2 is measured six times in order to verify the
stability of the value and to preclude a subsequent creep reaction.
10. Pipette the reagent into the blank value and sample cuvettes
according to the process specification.
11. Press READ CAROUSEL.
The 15:00 timer starts. When the time elapses, two short acoustic
signals are sounded.
Extinction 3 is measured in all cuvettes.
The result is indicated in g/L D-glucose, g/L D-fructose,
g/100 g D-glucose, g/100 g D-fructose on the display.
Program number 2210 2211
Parameter
D-glucose/
D-fructose D-glucose/
D-fructose (S)
R-Biopharm AG order number Art. no. 10 139 106 035
Results information
g D-glucose / L sample solution
g D-fructose / L sample solution
g D-glucose / 100 g sample
g D-fructose / 100 g sample
Carousel loading
Carousel position 1: blank value
Carousel position 2-7: Samples 1 to 6