Hach-Lange LZV937 - DR 6000 additional software enzymology tests - Handbuch (Seite 41)

English 41
D-isocitric acid and D-isocitric acid (S)
Procedure for D-isocitric acid
1. Prepare the test according to the process specification.
2. Select STORED PROGRAMS > SELECT BY NUMBER.
3. Enter the number 2214 for the program "D-isocitric acid". Confirm
with OK.
Enter the number 2215 for the program "D-isocitric acid (S)" with
creep monitoring. Confirm with OK.
4. Press START to start the program.
5. Set the blank value cuvette to carousel position 1.
6. Set the sample cuvettes to carousel positions 2-7.
7. Press READ CAROUSEL.
The 3:00 timer starts. When the time elapses, two short acoustic
signals are sounded.
Extinction 1 is measured in all cuvettes. R1 is shown on the display.
8. Pipette the reagent into the blank value and sample cuvettes
according to the process specification.
9. Press READ CAROUSEL.
The 10:00 timer starts. When the time elapses, two short acoustic
signals are sounded.
Extinction 2 is measured in all cuvettes.
Note: For program 2215 D-isocitric acid (S) with creep monitoring,
extinction 2 is measured six times in order to verify the stability of
the value and to preclude a subsequent creep reaction.
The result is indicated in g/L D-isocitric acid and g/100 g D-isocitric
acid on the display.
Program number 2214 2215
Parameter D-isocitric acid D-isocitric acid (S)
R-Biopharm AG order number Art. no. 10 414 433 035
Results information
g D-isocitric acid / L sample solution
g D-isocitric acid / 100 g sample
Carousel loading
Carousel position 1: Blank value
Carousel position 2-7: Samples 1 to 6