3 seq stepper, 4 mix + dilute, 3 seq stepper 4.7.4 mix + dilute – Hach-Lange TENSETTE plus User Manual Benutzerhandbuch
Seite 24

Operation
24
4.7.3 SEQ STEPPER
The sequential stepper mode enables serial dispensing of different
volumes (in normal stepper mode only fixed volume).
1.
Select PROGRAM
→ EDIT, then select with the scroll key
SEQ STEPPER and accept with OK.
2.
Then select the amount of dispensings (maximum 20) with the
scroll key and accept with OK. VOL 1 is displayed and the
highest possible volume is flashing.
3.
Select the first volume with the scroll key and accept with OK.
Now VOL 2 is displayed and the highest possible volume is
flashing.
4.
Select the second volume with the scroll key and accept with
OK. After selecting the last volume the total volume is shown on
the display and speed-in is flashing. Select the pipetting speeds
and the pipette is ready for pipetting.
5.
Fill a clean reagent reservoir with the liquid to be dispensed.
6.
Dip the tip under the surface of the liquid in the reservoir and
press the trigger. This action will fill the tip and the first volume is
displayed.
7.
Withdraw the tip from the liquid touching it against the edge of
the reservoir to remove excess liquid.
8.
Deliver the first volume by pressing the trigger. The display
shows the next volume.
9.
Continue dispensing by repeating step 3. After the last step
BLOWOUT is displayed.
10. To empty the tip completely, press the trigger.
Change the tip and continue pipetting.
Note: The pipette can be emptied at any time by pressing CANCEL (left
selection key
Figure 2 Pipette operation on page 15
).
4.7.4 MIX + DILUTE
1.
Select PROGRAM
→ EDIT, then select with the scroll key
MIX + DILUTE and accept with OK.
2.
Select the pipetting first volume by pressing the scroll key up or
down.
3.
Accept the volume with OK.
4.
Next select the second volume (VOL 2) with the scroll key and
accept with OK.
5.
Then select the pipetting speeds.
6.
Fill a clean reagent reservoir with the liquid to be dispensed.
7.
Dip the tip under the surface of the first liquid in the reservoir and
press the trigger. The first volume is drawn in to the tip. AIR is
displayed.