4 adjustment, 1 two point calibration – Hach-Lange TENSETTE plus User Manual Benutzerhandbuch
Seite 28
Calibration
28
Procedure:
1.
Do 10 pipettings with the minimum volume.
2.
Do 10 pipettings with the maximum volume.
3.
Calculate the accuracy (A) and precision (cv) of both series.
4.
Compare the results to the limits in the
If the calculated results are within the selected limits, the adjustment
of the pipette is correct.
5.4 Adjustment
5.4.1 Two point calibration
Normally the two point calibration should be used.
1.
Do the pipetting series with both maximum and minimum
volumes.
2.
Calculate the actual results.
3.
Select MENU and select OPTIONS with the scroll key and
accept with OK.
4.
Select CALIBRATE and accept with OK.
5.
Select TWO POINTS and confirm with OK. The target maximum
and target minimum volumes are shown on the display.
6.
Select EDIT and change the actual maximum volume with the
scroll key and accept with OK.
7.
Change the actual minimum volume with the scroll key and
accept with OK.
8.
A text SAVE? is displayed.
9.
Accept with YES.
10. The adjustment has been changed.
Table 2 Maximum permissible errors according ISO 8655
Range
Volume
Accuracy
Precision
µL
µL
%
s.d. µL
cv%
0.2–5 mL
5000
±40.0
±0.8
15.0
0.3
200 ±40.0
±20.0
15.0
7.5