Hach-Lange LZV936 - DR 6000 Brewery Analysis software - Handbuch (Seite 45)

English 45
Determination of the calibration curve
The factor 1 = 0.037 is an empirical factor and must be individually
determined through a calibration curve. The factor is the slope of the
calibration curves.
1. Pipette 40 mL of beer into four individual 50 mL volumetric flasks.
2. Pipette into the volumetric flasks each0.40 mL, 0.80 mL, 1.60 mL
and 3.20 mL of the iron standard solution (5 mg Fe
3+ /mL).
3. Add to each volumetric flask 2 mL ferrozine reagent and 1 mL
ascorbic acid solution.
4. Fill to the mark with dist. H
2
O.
5. Measure the extinction of the solution in a 40 mm rectangular
cuvette at 560 nm against a corresponding blank value.
Subtract the results of the un-spiked sample from the spiked samples.
Using these programs with the SIP 10 sipper
Information on the installation, configuration, and sample insertion when
using the SIP 10 sipper can be found in the SIP 10 user manual.
Accessories
Description Order no.
Rectangular cuvettes set (standard deviation = 10 mm,
aligned pair)
2095100
Rectangular cuvette QS, standard deviation = 10 mm
(3.5 mL)
2624410
Rectangular cuvette OS, standard deviation = 10 mm
(3 units)
LZP045
Rectangular cuvette QS, standard deviation = 20 mm LZV008
Rectangular cuvette OS, standard deviation = 20 mm LZP331
Rectangular cuvette QS, standard deviation = 50 mm
(17.5 mL)
2624450
Rectangular cuvette OS, standard deviation = 50 mm LZP167
Pour-through cuvette QS,
Standard deviation = 3 mm, center height = 10 mm, height =
40 mm LZV638
Pour-through cuvette QS,
Standard deviation = 50 mm, center height = 10 mm, height
= 40 mm LZV649
Pour-through cuvette QS,
Standard deviation = 10 mm, center height = 10 mm, height
= 40 mm LZV510
SIP 10 sipper module set for DR 6000 complete with tray,
tube set and 1 cm quartz glass pour-through cuvette, EU
LQV157.99.30001