3 seq stepper, 4 mix + dilute, 3 seq stepper 4.7.4 mix + dilute – Hach-Lange TENSETTE plus User Manual Benutzerhandbuch

Seite 24

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Operation

24

4.7.3 SEQ STEPPER

The sequential stepper mode enables serial dispensing of different

volumes (in normal stepper mode only fixed volume).

1.

Select PROGRAM

EDIT, then select with the scroll key

SEQ STEPPER and accept with OK.

2.

Then select the amount of dispensings (maximum 20) with the

scroll key and accept with OK. VOL 1 is displayed and the

highest possible volume is flashing.

3.

Select the first volume with the scroll key and accept with OK.

Now VOL 2 is displayed and the highest possible volume is

flashing.

4.

Select the second volume with the scroll key and accept with

OK. After selecting the last volume the total volume is shown on

the display and speed-in is flashing. Select the pipetting speeds

and the pipette is ready for pipetting.

5.

Fill a clean reagent reservoir with the liquid to be dispensed.

6.

Dip the tip under the surface of the liquid in the reservoir and

press the trigger. This action will fill the tip and the first volume is

displayed.

7.

Withdraw the tip from the liquid touching it against the edge of

the reservoir to remove excess liquid.

8.

Deliver the first volume by pressing the trigger. The display

shows the next volume.

9.

Continue dispensing by repeating step 3. After the last step

BLOWOUT is displayed.

10. To empty the tip completely, press the trigger.
Change the tip and continue pipetting.

Note: The pipette can be emptied at any time by pressing CANCEL (left
selection key

Figure 2 Pipette operation on page 15

, item

6

).

4.7.4 MIX + DILUTE

1.

Select PROGRAM

EDIT, then select with the scroll key

MIX + DILUTE and accept with OK.

2.

Select the pipetting first volume by pressing the scroll key up or

down.

3.

Accept the volume with OK.

4.

Next select the second volume (VOL 2) with the scroll key and

accept with OK.

5.

Then select the pipetting speeds.

6.

Fill a clean reagent reservoir with the liquid to be dispensed.

7.

Dip the tip under the surface of the first liquid in the reservoir and

press the trigger. The first volume is drawn in to the tip. AIR is

displayed.

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