Hach-Lange TENSETTE plus User Manual - Handbuch (Seite 26)

Operation
26
4.7.6 SEQ + ASPIRATE
The sequential aspirate mode enables serial aspirating of different
volumes.
1. Select PROGRAM → EDIT, then select with the scroll key
SEQ + ASPIRATE and accept with OK.
2. First choose the number of volumes (maximum 20) with the
scroll key and accept with OK. VOL 1 is displayed and the
highest possible volume is flashing.
3. Select the first volume with the scroll key and accept with OK.
Now VOL 2 is displayed and the highest possible remaining
volume is flashing.
4. Select the second volume with the scroll key and accept with
OK. After selecting the last volume the total volume is shown on
display and speed-in is flashing for selecting the pipettings
speeds. After selecting the speeds the first volume is shown on
the display and the pipette is ready for pipetting.
5. Fill a clean reagent reservoir with the liquid to be dispensed.
6. Dip the tip under the surface of the liquid and press the trigger.
This action will take up first volume and the next volume is
displayed.
7. Withdraw the tip from the liquid touching it against the edge of
the reservoir to remove excess liquid.
8. Dip the tip under the surface of the next liquid and press the
trigger. This action will take up the current volume and the next
volume appears on the display.
9. Repeat steps 2. and 3. until last volume is drawn in to the tip. The
total volume is shown on the display.
10. Deliver the total volume by pressing the trigger and hold it down
to empty the tip.
11. Release the trigger to return to the ready position.
Change the tip and continue pipetting.
Note: The pipette can be emptied at any time by pressing CANCEL (left
selection key
Figure 2 Pipette operation on page 15 , item 6 )