Metrohm tiamo 1.2 Manual - Handbuch (Seite 560)

534 Method
[ Dosing ]
The given volume is added. No automatic filling takes place either before or
after.
Fill
Filling the cylinder can take place from a freely selectable port. The valve
disk then remains at the selected port.
Aspirate
With this function liquid is aspirated via the given port. As with Dosing , the
cylinder is not automatically filled either before or after the command. It
should be possible for the aspirated volume to be removed with a single
piston stroke.
Eject to stop
With this function the whole cylinder content is ejected via the given port.
The piston is lowered to the stop point i.e. past the maximum volume mark.
This function should only be carried out to eject any air bubbles.
Eject to end volume
The whole cylinder content is ejected via the given port. In contrary to the
function
Eject to stop the piston is lowered only to the maximum volume
mark i.e. 10'000 pulses. This function should be used for pipetting se-
quences to empty the cylinder.
Exchange position
With this function the cylinder is filled via the given port first. Afterwards, the
valve disk is switched to
port 2 , the dosing drive can then be removed from
the dosing unit.
Change port
The valve disk is rotated to the given Port. No piston movement takes
place.
Compensate
As the dosing units are exchangeable, the coupling of the Dosino piston
rod (spindle) has a slight mechanical tolerance that can be noticed when
the piston changes its direction of movement. This tolerance can be com-
pensated with this function. A short piston movement is first made in the
same direction as the previous movement; this is followed by a piston
movement in the reverse direction.
Port
1 ... 4
Default values:
1 : for Dosing , Aspirate
2 : for Fill , Exchange position , Change port
4 : for Eject to stop , Eject to end volume , Compensate
The desired port for the selected function has to be defined for each liquid
handling command.